Modern Techniques for the Isolation of Extracellular Vesicles and Viruses
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of Faecalibacterium prausnitzii extracellular vesicles
Introduction: Extracellular vesicles (EVs) contain active biological compounds which play important roles in biological processes. The secretion of EVs is a common phenomenon which occurs in archaea, bacteria and mammalian cells. The secretion of bacterial EVs has been discovered in various species of both Gram-negative and Gram-positive bacteria. Faecalibacterium prausnitzii is one of the comm...
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Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype betwee...
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چکیده ندارد.
15 صفحه اولCurrent methods for the isolation of extracellular vesicles.
Extracellular vesicles (EVs), including microvesicles and exosomes, are nano- to micron-sized vesicles, which may deliver bioactive cargos that include lipids, growth factors and their receptors, proteases, signaling molecules, as well as mRNA and non-coding RNA, released from the cell of origin, to target cells. EVs are released by all cell types and likely induced by mechanisms involved in on...
متن کاملTechniques for the analysis of extracellular vesicles using flow cytometry.
Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help regulate a diverse range of biological processes. Analysis of EVs using flow cytometry (FCM) has been notoriously difficult due to their small size and lack of discrete populations positive for markers of interest. Methods for EV analysis, while c...
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ژورنال
عنوان ژورنال: Journal of Neuroimmune Pharmacology
سال: 2019
ISSN: 1557-1890,1557-1904
DOI: 10.1007/s11481-019-09874-x